Abstract The agarophyte Gracilariopsis lemaneiformis is both important for biological research and of significant economic value. However, the genetic diversity of wild populations
of the alga has not been studied. We used amplified fragment length polymorphism (AFLP) PCR and simple sequence repeat (SSR)
analysis to investigate diversity in four field populations, three from the coast of Qingdao and one from Weihai, China. Forty
G. lemaneiformis isolates collected from the four different geographical groups were analyzed using 16 pairs of SSR primers for PCR amplification.
However, no polymorphisms were detected, indicative of a degree of genetic homogeneity. A total of 347 reproducible bands
were then amplified using eight AFLP primer pairs, and genetic indices of diversity within and between populations were calculated.
This analysis revealed only low levels of genetic diversity both within and between the four geographical groups of G. lemaneiformis. The Weihai population showed a higher within-population genetic diversity than any of the Qingdao populations. The data
suggest that there is only limited gene flow between populations. An UPGMA dendrogram revealed two main clusters, and one
of these included all the Qingdao isolates. The order of clustering was in accordance with their geographical distribution.
These results suggest that the wild G. lemaneiformis populations are closely related and that there is little genetic diversity within wild germplasm in the regions sampled.
of the alga has not been studied. We used amplified fragment length polymorphism (AFLP) PCR and simple sequence repeat (SSR)
analysis to investigate diversity in four field populations, three from the coast of Qingdao and one from Weihai, China. Forty
G. lemaneiformis isolates collected from the four different geographical groups were analyzed using 16 pairs of SSR primers for PCR amplification.
However, no polymorphisms were detected, indicative of a degree of genetic homogeneity. A total of 347 reproducible bands
were then amplified using eight AFLP primer pairs, and genetic indices of diversity within and between populations were calculated.
This analysis revealed only low levels of genetic diversity both within and between the four geographical groups of G. lemaneiformis. The Weihai population showed a higher within-population genetic diversity than any of the Qingdao populations. The data
suggest that there is only limited gene flow between populations. An UPGMA dendrogram revealed two main clusters, and one
of these included all the Qingdao isolates. The order of clustering was in accordance with their geographical distribution.
These results suggest that the wild G. lemaneiformis populations are closely related and that there is little genetic diversity within wild germplasm in the regions sampled.
- Content Type Journal Article
- DOI 10.1007/s10811-009-9500-3
- Authors
- Qiaoqiao Pang, Ocean University of China Key Laboratory of Marine Genetics and Breeding, Ministry of Education Qingdao 266003 People’s Republic of China
- Zhenghong Sui, Ocean University of China Key Laboratory of Marine Genetics and Breeding, Ministry of Education Qingdao 266003 People’s Republic of China
- Kyoung Ho Kang, Chonnam National University Department of Aquaculture Yeosu 550-749 Jeonnam South Korea
- Fanna Kong, Ocean University of China Key Laboratory of Marine Genetics and Breeding, Ministry of Education Qingdao 266003 People’s Republic of China
- Xuecheng Zhang, Ocean University of China Key Laboratory of Marine Genetics and Breeding, Ministry of Education Qingdao 266003 People’s Republic of China
- Journal Journal of Applied Phycology
- Online ISSN 1573-5176
- Print ISSN 0921-8971
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