Seasonal changes in proteomic profiles of Japanese kelp: Saccharina japonica (Laminariales, Phaeophyceae)

Seasonal changes in proteomic profiles of Japanese kelp: Saccharina japonica (Laminariales, Phaeophyceae): "

Abstract The seasonal variation in protein expression in the sporophyte of Saccharina japonica (Areschoug) Lane, Mays, Druehl and Saunders was investigated. High-quality proteins that are available for protein profiling
were extracted by the ethanol/phenol extraction method, and 564 protein spots in total were detected. Proteins were identified
through database search by combining Mascot and MS BLAST for 100 spots, and significant difference of expression level between
the samples collected in winter and in summer was observed in the case of 95 spots. Within 67 spots upregulated in the samples
collected in summer, vanadium-dependent bromoperoxidase (vBPO) were identified for 21spots. It is thought that the elevation
of expression level of vBPO in summer depend on the activation of the functions: (1) elimination of active oxygen species
and protection of the algal body from oxygen injury, (2) prevention of the growth inhibition due to the adherence of attached
organisms, in the season.

• Content Type Journal Article
• DOI 10.1007/s10811-009-9477-y
• Authors
  
  
  • Norishige Yotsukura, Hokkaido University Field Science Center for Northern Biosphere Sapporo Hokkaido 060-0809 Japan
  • Kouhei Nagai, St. Marianna University School of Medicine Kawasaki Kanagawa 216-8514 Japan
  • Hajime Kimura, Fisheries Experimental Station Kushimoto Wakayama 649-3503 Japan
  • Kouichi Morimoto, Kinki University Department of Biotechnological Science Kinokawa Wakayama 649-6493 Japan
  
  

• Journal Journal of Applied Phycology
• Online ISSN 1573-5176
• Print ISSN 0921-8971

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