Carotenoid fluorescence in Dunaliella salina

Carotenoid fluorescence in Dunaliella salina: "

Abstract

Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly
stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine
and/or localise the carotenoid globules in vivo. In this study, we show that the carotenoid-rich globules emit clear green
fluorescence, which can be used in, for example, fluorescence microscopy (e.g. CLSM) to obtain pictures of the cells and their
carotenoid content.

• Content Type Journal Article
• DOI 10.1007/s10811-010-9505-y
• Authors
  
  
  • Dorinde M. M. Kleinegris, Wageningen University and Research Centre Agrotechnology and Food Science, Bioprocess Engineering P.O. Box 8129 6700 EV Wageningen The Netherlands
  • Marjon A. van Es, Wageningen University and Research Centre Agrotechnology and Food Science, Bioprocess Engineering P.O. Box 8129 6700 EV Wageningen The Netherlands
  • Marcel Janssen, Wageningen University and Research Centre Agrotechnology and Food Science, Bioprocess Engineering P.O. Box 8129 6700 EV Wageningen The Netherlands
  • Willem A. Brandenburg, Wageningen University and Research Centre Plant Research International BV, Agrosystems Research P.O. Box 16 6700 AV Wageningen The Netherlands
  • René H. Wijffels, Wageningen University and Research Centre Agrotechnology and Food Science, Bioprocess Engineering P.O. Box 8129 6700 EV Wageningen The Netherlands
  
  

• Journal Journal of Applied Phycology
• Online ISSN 1573-5176
• Print ISSN 0921-8971

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