Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica

Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica: "

Abstract Expressed sequence tag-derived microsatellite markers (EST-SSR) were generated and characterized in Laminaria japonica using data mining from updated public EST databases and polymorphism testing. Fifty-eight of 578 ESTs (10.0%) containing
various repeat motifs were used to design polymerase chain reaction (PCR) amplification primers. A total of 12 pairs of primer
were generated and used in the PCR amplification. Alleles per locus ranged from two to ten (average of 5.7). The observed
heterozygosities and expected heterozygosities were from 0.045 to 0.543 and from 0.056 to 0.814, respectively. All loci were
in Hardy–Weinberg equilibrium and no linkage disequilibrium was detected. These robust, informative, and potentially transferable
polymorphic markers appear suitable for population, genetic, parentage, and mapping studies of L. japonica.

• Content Type Journal Article
• DOI 10.1007/s10811-009-9426-9
• Authors
  
  
  • Fuli Liu, Chinese Academy of Sciences Institute of Oceanology Qingdao 266071 China
  • Xiuliang Wang, Chinese Academy of Sciences Institute of Oceanology Qingdao 266071 China
  • Jianting Yao, Chinese Academy of Sciences Institute of Oceanology Qingdao 266071 China
  • Wandong Fu, Chinese Academy of Sciences Institute of Oceanology Qingdao 266071 China
  • Delin Duan, Chinese Academy of Sciences Institute of Oceanology Qingdao 266071 China
  
  

• Journal Journal of Applied Phycology
• Online ISSN 1573-5176
• Print ISSN 0921-8971

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