Abstract
A rapid, sensitive algal bioassay was investigated to monitor copper toxicity using photosynthetic activity and motility parameters
as end points in the photosynthetic flagellate Euglena gracilis. Effects on motility parameters were determined using the automatic bioassay ECOTOX. Photosynthetic efficiency was measured
using chlorophyll fluorescence by means of a fast, noninvasive pulse amplitude modulated fluorometer. These parameters were
assessed regarding their effectiveness as end points for short- (0–24 h) and long-term (1–5 days) toxicity tests. The model
organism showed significant responses to the tested concentrations at both motility and photosynthesis examined levels. EC50 values for movement parameters immediately after the cells were mixed with copper are 19.09 (300.4 μM), 20.4 (312 μM) and
23.09 (363.35 μM) mg L−1 for motility, r-value and velocity, respectively. The current study proofs that Euglena is a convenient biotest organism where motility parameters show rapid sensitive responses to copper (direct exposure to copper).
Also, the photosynthetic parameters appear to be appropriate for acute and chronic toxicity tests for higher and lower copper
concentrations, respectively.
as end points in the photosynthetic flagellate Euglena gracilis. Effects on motility parameters were determined using the automatic bioassay ECOTOX. Photosynthetic efficiency was measured
using chlorophyll fluorescence by means of a fast, noninvasive pulse amplitude modulated fluorometer. These parameters were
assessed regarding their effectiveness as end points for short- (0–24 h) and long-term (1–5 days) toxicity tests. The model
organism showed significant responses to the tested concentrations at both motility and photosynthesis examined levels. EC50 values for movement parameters immediately after the cells were mixed with copper are 19.09 (300.4 μM), 20.4 (312 μM) and
23.09 (363.35 μM) mg L−1 for motility, r-value and velocity, respectively. The current study proofs that Euglena is a convenient biotest organism where motility parameters show rapid sensitive responses to copper (direct exposure to copper).
Also, the photosynthetic parameters appear to be appropriate for acute and chronic toxicity tests for higher and lower copper
concentrations, respectively.
- Content Type Journal Article
- DOI 10.1007/s10811-010-9520-z
- Authors
- Hoda Ahmed, Friedrich-Alexander Universität Department für Biologie Staudtstr. 5 91058 Erlangen Germany
- Donat-Peter Häder, Friedrich-Alexander Universität Department für Biologie Staudtstr. 5 91058 Erlangen Germany
- Journal Journal of Applied Phycology
- Online ISSN 1573-5176
- Print ISSN 0921-8971
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