Abstract We report a procedure for obtaining fairly pure phycocyanin from a local isolate of the cyanobacterium Synechococcus sp (Anacystis nidulans BD1). Cells were incubated with 1 mg∙mL−1 of lysozyme at 37°C for 16 h with shaking. The cell-free extract was treated with activated charcoal and chitosan. The purity
(A
620/280) of phycocyanin obtained after lysozyme treatment was up to 2.18, which could be improved to 4.72 after incubation with activated
charcoal and chitosan. The yield of phycocyanin was 80–100 mg∙g−1 dry weight of cells. The method reported here is a single-step and efficient procedure and has the potential to be adopted
for large-scale production of phycocyanin.
(A
620/280) of phycocyanin obtained after lysozyme treatment was up to 2.18, which could be improved to 4.72 after incubation with activated
charcoal and chitosan. The yield of phycocyanin was 80–100 mg∙g−1 dry weight of cells. The method reported here is a single-step and efficient procedure and has the potential to be adopted
for large-scale production of phycocyanin.
- Content Type Journal Article
- DOI 10.1007/s10811-009-9449-2
- Authors
- Alka Gupta, Bhabha Atomic Research Centre Molecular Biology Division Mumbai 400 085 India
- Jayashree K. Sainis, Bhabha Atomic Research Centre Molecular Biology Division Mumbai 400 085 India
- Journal Journal of Applied Phycology
- Online ISSN 1573-5176
- Print ISSN 0921-8971
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