Abstract Beneficial effects of seaweeds and their extracts on crop performance have been attributed to a variety of compounds, including
the betaines which are quaternary ammonium betaines. Methods of analysis of betaines published thus far suffer from low sensitivity,
lack of baseline separation of individual betaines and from interference from other sample constituents. A rapid cleanup protocol
and a sensitive LC-MS/MS method of analysis were developed to afford baseline separation of four betaines in the brown alga
Ascophyllum nodosum and its commercial seaweed extract. Using this method, the presence of glycine betaine, δ-aminovaleric acid betaine, γ-aminobutyric
acid betaine and laminine in A. nodosum, and commercial extracts derived from A. nodosum, were confirmed and quantified. The major betaine present was γ-aminobutyric acid betaine accounting for 0.008–0.014% of
the dry weight of the seaweed and 0.014–0.027% of the dry weight of the commercial extracts. Seasonal variation in betaine
content was observed. Differences in the total betaine content were observed between A. nodosum of the yellow (0.011–0.017% dry weight) and the olive green (0.017–0.021% dry weight) coloured morphologies.
the betaines which are quaternary ammonium betaines. Methods of analysis of betaines published thus far suffer from low sensitivity,
lack of baseline separation of individual betaines and from interference from other sample constituents. A rapid cleanup protocol
and a sensitive LC-MS/MS method of analysis were developed to afford baseline separation of four betaines in the brown alga
Ascophyllum nodosum and its commercial seaweed extract. Using this method, the presence of glycine betaine, δ-aminovaleric acid betaine, γ-aminobutyric
acid betaine and laminine in A. nodosum, and commercial extracts derived from A. nodosum, were confirmed and quantified. The major betaine present was γ-aminobutyric acid betaine accounting for 0.008–0.014% of
the dry weight of the seaweed and 0.014–0.027% of the dry weight of the commercial extracts. Seasonal variation in betaine
content was observed. Differences in the total betaine content were observed between A. nodosum of the yellow (0.011–0.017% dry weight) and the olive green (0.017–0.021% dry weight) coloured morphologies.
- Content Type Journal Article
- DOI 10.1007/s10811-009-9483-0
- Authors
- Shawna L. MacKinnon, National Research Council of Canada Institute for Marine Biosciences 1411 Oxford Street Halifax NS Canada B3H 3Z1
- David Hiltz, Acadian Seaplants Limited 30 Brown Ave. Dartmouth NS Canada B3B 1X8
- Raul Ugarte, Acadian Seaplants Limited 30 Brown Ave. Dartmouth NS Canada B3B 1X8
- Cheryl A. Craft, National Research Council of Canada Institute for Marine Biosciences 1411 Oxford Street Halifax NS Canada B3H 3Z1
- Journal Journal of Applied Phycology
- Online ISSN 1573-5176
- Print ISSN 0921-8971
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