Abstract We developed a rapid and effective procedure for scanning electron microscopy of three delicate dinoflagellates, Karlodinium micrum, Akashiwo sanguinea, and Heterocapsa niei. Good results were obtained when the specimens were fixed with a modified Párducz’s fixative (2% osmium tetroxide:saturated
mercuric chloride = 5:1 v/v) for 10 min, washed in 0.05 M sodium cacodylate trihydrate buffer for 2 min, dehydrated in tert-butanol for 10 min and dried with hexamethyldisilazane in air for 3 min in a fume hood because reagents are very toxic. This
method could be completed in 25 min. Compared with other preparative techniques, the present protocol has significant advantages
for SEM observation by limiting distortion of delicate specimens and reducing the preparation time.
mercuric chloride = 5:1 v/v) for 10 min, washed in 0.05 M sodium cacodylate trihydrate buffer for 2 min, dehydrated in tert-butanol for 10 min and dried with hexamethyldisilazane in air for 3 min in a fume hood because reagents are very toxic. This
method could be completed in 25 min. Compared with other preparative techniques, the present protocol has significant advantages
for SEM observation by limiting distortion of delicate specimens and reducing the preparation time.
- Content Type Journal Article
- DOI 10.1007/s10811-009-9461-6
- Authors
- Seung Won Jung, Korea Ocean Research and Development Institute South Sea Institute Geoje 656-830 Republic of Korea
- Hyoung Min Joo, Sangmyung University Department of Life Science Seoul 110-743 Republic of Korea
- Joon Sang Park, Sangmyung University Department of Life Science Seoul 110-743 Republic of Korea
- Jin Hwan Lee, Sangmyung University Department of Life Science Seoul 110-743 Republic of Korea
- Journal Journal of Applied Phycology
- Online ISSN 1573-5176
- Print ISSN 0921-8971
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